Culture & Sensitivity, Aerobic bacteria Body Fluids Specify type
Report in 24Hrs
At Home
Details
Identify aerobic bacterial pathogens causing infections in sterile body cavities
₹299₹1600
81% OFF
1000+ Booked this Test
🧪 Culture & Sensitivity – Aerobic Bacteria (Body Fluids)
🧫 Sample types: Pleural, Ascitic, Pericardial, Synovial, CSF, Peritoneal Dialysate, etc.
🔍 Overview
This test is performed to detect aerobic bacterial infections in sterile body fluids and to determine the antibiotic sensitivity of any isolated organisms. Early detection is critical to prevent complications like sepsis or organ failure.
📌 Test Details
Parameter | Description |
|---|---|
Sample Type | Body fluid – must specify the source (e.g., pleural, ascitic, CSF, synovial) |
Collection Method | Sterile aspiration (paracentesis, thoracentesis, lumbar puncture, etc.) |
Fasting Required | ❌ Not required |
Volume Required | Minimum 2–5 mL (depending on the type of fluid) |
Turnaround Time | Preliminary: 24–48 hours |
Departments Involved | Microbiology & Infectious Disease |
🎯 Purpose of the Test
- Identify aerobic bacterial pathogens causing infections in sterile body cavities
- Perform antibiotic sensitivity testing (AST) to guide precise treatment
- Distinguish between infective and non-infective inflammation
🦠 Common Organisms Detected
Fluid Type | Possible Aerobic Pathogens |
|---|---|
Pleural Fluid | Streptococcus pneumoniae, Staph aureus, E. coli |
Ascitic Fluid | E. coli, Klebsiella, Enterococcus |
CSF (Meningitis) | Neisseria meningitidis, H. influenzae, Strep pneumoniae |
Synovial Fluid | Staph aureus, Strep spp., Pseudomonas |
Pericardial Fluid | Mycobacterium tuberculosis (via other culture) + aerobic flora |
Peritoneal Dialysate | Staph epidermidis, Enterococcus |
📈 Interpreting the Results
Result | Interpretation |
|---|---|
✅ No Growth | No aerobic organisms detected |
⚠️ Positive Growth | Infection detected; organism identification + antibiotic sensitivity to follow |
❌ Contaminant | Rare, but possible if aseptic technique not followed during sample collection |
💊 Sensitivity Testing (AST)
Determines the antibiotics that the bacteria are:
- ✅ Sensitive (S) – effective
- ⚠️ Intermediate (I) – may respond partially
- ❌ Resistant (R) – not effective
Helps tailor antibiotic therapy and avoid resistance.
🛑 Pre-Test Guidelines
Guideline | Importance |
|---|---|
Use sterile technique | Prevent contamination |
Label fluid type clearly | Guides culture media and incubation conditions |
Collect before antibiotics | Reduces false negatives |
Transport immediately | Prevents overgrowth or organism death |
🧪 Recommended Additional Tests
Alongside Culture | Purpose |
|---|---|
Gram Stain / ZN stain | Preliminary identification of organism type |
Cell count & differential | Evaluate inflammation (WBCs, RBCs, PMNs) |
Protein, glucose, LDH | Assess exudate vs transudate nature |
AFB culture / TB PCR | If TB is suspected (esp. pleural/ascitic fluid) |
Fungal Culture | In immunocompromised or chronic infections |
🧠 Summary Table
Aspect | Details |
|---|---|
Sample | Body fluid (e.g., CSF, pleural, ascitic) – specify source |
Tests For | Aerobic bacteria and antibiotic sensitivity |
Used To Diagnose | Meningitis, pleuritis, peritonitis, synovitis, pericarditis, etc. |
Next Steps | Tailored antibiotic treatment |
How our test process works!
