Culture & Sensitivity, Aerobic bacteria Other samples
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Identifies aerobic bacterial infections from non-routine clinical samples.It also determines which antibiotics are most effective against the detected organism
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🧪 Culture & Sensitivity – Aerobic Bacteria (Other Samples)
🔍 Overview
This test identifies aerobic bacterial infections from non-routine clinical samples such as wound swabs, pus, tissue biopsies, throat swabs, sputum, ear or eye discharge, skin lesions, vaginal swabs, or any site-specific exudate. It also determines which antibiotics are most effective against the detected organism.
📦 Sample Details
Parameter | Details |
|---|---|
Sample Type | Wound swab, pus, eye/ear/nasal discharge, sputum, skin scrapings, etc. |
Volume/Amount Required | Depends on sample (e.g., swab, 1–2 mL pus/fluid, tissue fragment) |
Collection Method | Aseptic collection in sterile containers or transport media |
Fasting Required | ❌ No |
Transport Time | Preferably within 1–2 hours |
Processing Time | 24–48 hrs for preliminary report |
Lab Department | Microbiology |
🎯 Purpose of the Test
Goal | Application |
|---|---|
Identify aerobic bacteria | Causing local or systemic infections |
Guide antibiotic therapy | Based on organism’s susceptibility |
Prevent inappropriate treatment | Avoid resistance and reduce complications |
Monitor chronic/recurrent cases | Especially in diabetics, immunocompromised |
🦠 Common Pathogens Detected
Sample Site | Likely Bacteria |
|---|---|
Wound / Ulcer / Pus | Staphylococcus aureus, Pseudomonas, E. coli, Proteus |
Ear Discharge | Pseudomonas aeruginosa, Staph aureus |
Eye Discharge | Streptococcus pneumoniae, Haemophilus |
Throat Swab | Strep pyogenes, Neisseria, Corynebacterium |
Sputum | Klebsiella, Streptococcus pneumoniae, H. influenzae |
Vaginal Swab | Gardnerella, E. coli, Enterococcus, Staph |
Skin/Nail Scrapings | Staph aureus, occasionally fungi (requires fungal culture) |
🧬 Antibiotic Sensitivity Testing (AST)
Performed using Kirby-Bauer disk diffusion or automated systems, this assesses:
- Which antibiotics will work (Sensitive – S)
- May partially work (Intermediate – I)
- Will not work (Resistant – R)
This guides precise antimicrobial treatment.
📊 Interpreting Results
Result | Meaning |
|---|---|
✅ No Growth | No aerobic bacterial infection detected |
⚠️ Growth of Pathogen | Infection confirmed, therapy guided by sensitivity report |
❌ Contaminants | Possible if improper collection or non-sterile site sample used |
🧪 Recommended Additional Tests
Test | Purpose |
|---|---|
Gram Stain | Preliminary ID: Gram +ve / -ve and shape |
Fungal Culture | If fungal infection suspected (esp. in skin/nail/eye) |
AFB Stain / TB PCR | In chronic discharges or if TB is suspected |
CBC, ESR, CRP | Assess systemic inflammation or infection severity |
Blood Culture | If systemic spread (sepsis) is suspected |
Wound Biopsy (if deep) | Especially in chronic diabetic wounds |
🧠 Clinical Significance
Use Case | Why This Test is Critical |
|---|---|
Chronic non-healing ulcers | Rule out multidrug-resistant or hospital-acquired infections |
ENT infections (otitis, conjunctivitis) | Identify fastidious organisms and guide local treatment |
Sputum in COPD, pneumonia | Prevent misuse of broad-spectrum antibiotics |
Diabetic foot infections | Early, precise intervention can prevent amputation |
📌 Summary Table
Aspect | Details |
|---|---|
Sample Types | Swabs, pus, discharges, sputum, scrapings, etc. |
Tests For | Aerobic bacterial growth + antibiotic susceptibility |
Conditions Diagnosed | Wound infections, ENT infections, respiratory, skin/soft tissue infections |
Next Steps | Antibiotic adjustment, isolation precautions, further evaluation |
How our test process works!
