Culture & Sensitivity, Aerobic bacteria Pus
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It identifies the causative bacteria and determines the antibiotics to which the bacteria are sensitive, resistant, or intermediate
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🧪 Culture & Sensitivity – Aerobic Bacteria (Pus Sample)
🔍 Overview
This test is performed to detect aerobic bacterial infections in pus samples collected from abscesses, infected wounds, or post-surgical sites. It identifies the causative bacteria and determines the antibiotics to which the bacteria are sensitive, resistant, or intermediate, helping in precise and effective treatment planning.
📦 Test Details
Parameter | Details |
|---|---|
Sample Type | Pus (aspirated or swabbed from wound/abscess) |
Collection Method | Sterile swab or syringe aspiration |
Container | Sterile container or transport medium |
Fasting Required | ❌ No |
Processing Time | 24–48 hrs (preliminary report), 72 hrs to 7 days (final) |
Department | Microbiology |
🎯 Clinical Indications
Used when patient presents with signs of local infection, such as:
- Skin or soft tissue abscess
- Post-surgical wound infection
- Diabetic foot ulcer
- Furuncles or carbuncles
- Pressure sores
- Infected injuries or bites
🦠 Common Bacteria Identified
Organism | Clinical Relevance |
|---|---|
Staphylococcus aureus | Most common, including MRSA |
Pseudomonas aeruginosa | Found in burn wounds, moist skin infections |
Escherichia coli | Often from bowel perforation or surgical wounds |
Klebsiella pneumoniae | Nosocomial infections |
Proteus species | Chronic ulcers |
Enterococcus species | Post-operative infections |
Streptococcus pyogenes | Spreading cellulitis or necrotizing fasciitis |
💊 Antibiotic Sensitivity Testing (AST)
- Performed on isolated bacteria using:
- Disk diffusion (Kirby-Bauer method)
- Automated systems (VITEK, MicroScan)
- Reports bacteria’s response as:
- S (Sensitive)
- I (Intermediate)
- R (Resistant)
📊 Result Interpretation
Result | Meaning |
|---|---|
✅ No Growth | No aerobic organisms detected |
❌ Positive Culture | Indicates type of bacteria and suitable antibiotics |
⚠️ Contaminant Alert | Sample contamination possible due to improper collection |
🧪 Recommended Additional Tests
Test | Purpose |
|---|---|
Gram Stain | Preliminary bacteria identification |
AFB Stain/TB PCR | If TB abscess suspected (esp. in cold abscess) |
Fungal Culture | If response to antibacterial therapy is poor |
CBC, ESR, CRP | Assess systemic infection or inflammation |
Blood Culture | If signs of systemic infection (fever, chills) present |
Wound Biopsy | In chronic non-healing ulcers or diabetic foot |
🧠 Pre-Test Considerations
Guideline | Reason |
|---|---|
Clean wound before sample collection | Reduces surface contaminants |
Collect pus from depth of lesion | More representative of true infection |
Prefer aspiration over swab | Lower contamination, higher pathogen yield |
Avoid prior antibiotic use | Can yield false-negative culture |
🧠 Clinical Relevance
Early identification of pathogens helps in:
- Avoiding empirical broad-spectrum antibiotics
- Reducing hospital stay and complications
- Monitoring antimicrobial resistance (AMR)
📌 Summary
Aspect | Details |
|---|---|
Sample | Pus (aspirated or swabbed) |
Tests For | Aerobic bacterial identification & antibiotic response |
Key Uses | Abscesses, ulcers, wound infections |
Next Step | Tailor antibiotic therapy based on sensitivity report |
How our test process works!
