25 OH Vitamin D
Unit Test
Report in 16Hrs
At Home
No Fasting Required
Details
Assesses 25-hydroxyvitamin D levels to determine deficiency, affecting bone strength and immune function.
₹299₹1280
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Parameters
25-OH Vitamin D Test
| Aspect | Details |
|---|---|
| Fasting Required? | Not mandatory (fasting optional) |
| Sample Type | Blood (serum) |
| Main Organs Involved | Skin (synthesis), liver (conversion), kidneys (activation), bones |
| Test Purpose | Measures total Vitamin D level to evaluate deficiency, sufficiency, or toxicity |
What Is 25-OH Vitamin D?
- 25-hydroxyvitamin
D is the storage form of Vitamin D, made in the liver from
either:
- o Vitamin D3 (cholecalciferol) – synthesized in skin or from animal-based foods
- o Vitamin D2 (ergocalciferol) – from plant-based sources or supplements
- It reflects total body Vitamin D stores and has a longer half-life than the active form (1,25-dihydroxyvitamin D).
Reference Ranges (ng/mL)
| Level | Interpretation |
|---|---|
| <10 ng/mL | Severe deficiency |
| 10–20 ng/mL | Deficient |
| 20–30 ng/mL | Insufficient |
| 30–50 ng/mL | Optimal/Sufficient |
| >100 ng/mL | Possible toxicity |
Why It's Done
| Use Case | Why It Matters |
|---|---|
| Bone health monitoring | Prevents/monitors osteomalacia, osteoporosis, rickets |
| Suspected deficiency | In limited sun exposure, dark skin, elderly, infants |
| Supplement therapy monitoring | Track safety and efficacy of Vitamin D replacement |
| Parathyroid or calcium disorders | Assists in evaluating hyperparathyroidism, calcium malabsorption |
| Autoimmune/chronic diseases | Associated with fatigue, depression, muscle weakness |
Causes of Deficiency
- Lack of sunlight (indoor lifestyle, sunscreen use)
- Poor dietary intake
- Malabsorption (e.g., celiac, Crohn’s)
- Chronic kidney/liver disease
- Obesity (Vitamin D gets sequestered in fat)
- Certain medications (steroids, anticonvulsants)
Sources of Vitamin D
| Type | Examples |
|---|---|
| D3 (Cholecalciferol | Sunlight, fatty fish, egg yolk, fortified dairy |
| D2 (Ergocalciferol) | Fortified cereals, mushrooms exposed to UV |
Recommended Complementary Tests
| If 25-OH D Is Abnormal | Why Add It |
|---|---|
| Calcium, Phosphorus, ALP | Bone metabolism markers |
| Parathyroid Hormone (PTH | Evaluates cause of low/high Vitamin D |
| Magnesium | Affects Vitamin D activation |
| 1,25-dihydroxyvitamin D | Consider if kidney dysfunction or sarcoidosis |
Summary Table
| Test | Purpose | Optimal Range | Sample Type |
|---|---|---|---|
| 25-OH Vitamin D | Assess total body Vitamin D stores | 30–50 ng/mL | Blood (serum) |
Supplementation Guidelines (General)
| Deficiency Level | Suggested Dose (under medical advice) |
|---|---|
| Mild deficiency (20–30 ng/mL) | 1,000–2,000 IU/day of D3 |
| Moderate (10–20 ng/mL) | 2,000–4,000 IU/day for 8–12 weeks |
| Severe (<10 ng/mL) | 50,000 IU weekly for 6–8 weeks, then recheck |
(Always consult a physician before starting supplements)
How our test process works!
