Anti Ds-DNA

Blood

Report in 4Hrs

At Home

No Fasting Required

Details

The anti-dsDNA antibody test measures autoantibodies that target the body’s own double-stranded DNA.\nIt is highly specific for: Systemic Lupus Erythematosus (SLE)\nOften used for diagnosis, monitoring, and prognosis of SLE. These antibodies can trigger inflammation and damage in kidneys, skin, joints, brain, and blood vessels

₹299₹1,650

82% OFF

Anti-Ds-DNA (Anti-Double Stranded DNA) Antibody Test

Why is it done?
- Detects antibodies against double-stranded DNA (dsDNA), which are autoimmune markers indicating the body's immune system is attacking its own genetic material
- Primarily used to diagnose systemic lupus erythematosus (SLE) and differentiate it from other autoimmune conditions
- Monitors disease activity and severity in patients with established SLE
- Helps predict the risk of lupus nephritis (kidney inflammation) and other organ involvement
- Ordered when patients present with symptoms suggestive of SLE such as joint pain, rash, fever, or unexplained fatigue
- Used as part of routine monitoring in SLE patients to assess treatment response and disease progression
Normal Range
- Normal Result (Negative): Less than 1 IU/mL (International Units per milliliter) or reported as 'Negative' or 'Not Detected'
- Borderline/Weakly Positive: 1-10 IU/mL or reported as 'Borderline Positive' - may warrant repeat testing or further evaluation
- Positive Result: Greater than 10 IU/mL or reported as 'Positive' - indicates presence of anti-dsDNA antibodies
- Units: Typically measured in IU/mL (International Units per milliliter); some laboratories use Crithidia luciliae immunofluorescence or ELISA methods
- Negative Result (Normal): Indicates absence of anti-dsDNA antibodies, typically consistent with absence of SLE or other autoimmune disease
- Positive Result (Abnormal): Indicates presence of anti-dsDNA antibodies, highly specific for SLE diagnosis
Interpretation
- Negative Result: Makes SLE diagnosis less likely; however, approximately 30% of SLE patients may be seronegative for anti-dsDNA despite having active disease
- Positive Result: Highly specific for SLE (95-99% specificity), indicating probable SLE diagnosis when combined with clinical symptoms and other autoimmune markers
- High Titers: Levels significantly elevated (>50 IU/mL) correlate with active disease, increased risk of lupus nephritis, and more severe organ involvement
- Rising Titers: Increasing antibody levels over time in SLE patients may indicate impending disease flare or worsening organ involvement
- Falling Titers: Decreasing levels typically indicate good treatment response and disease remission in SLE patients
- Clinical Correlation Required: Results must be interpreted with patient symptoms, clinical findings, and other laboratory tests (ANA, complement levels, CBC)
- Factors Affecting Results: Disease activity, medications (immunosuppressants, corticosteroids), pregnancy, infections, and genetic predisposition can influence antibody levels
- Rare Positive Results: Anti-dsDNA may occasionally be positive in other conditions including drug-induced lupus, lupus-like syndromes, or other connective tissue diseases
Associated Organs
- Primary Organ Systems: Immune system (affected by autoimmunity), kidneys, joints, skin, heart, lungs, and nervous system
- Systemic Lupus Erythematosus (SLE): Most common disease associated with anti-dsDNA positivity; autoimmune disease affecting multiple organ systems
- Lupus Nephritis (Kidney): High anti-dsDNA titers strongly associated with kidney involvement; antibodies deposit in glomeruli causing inflammation and renal dysfunction; may lead to chronic kidney disease or end-stage renal disease if untreated
- Joint Involvement (Arthritis): Anti-dsDNA positive patients often experience arthritis and joint inflammation, typically affecting hands, wrists, and knees
- Cutaneous Manifestations (Skin): Characteristic malar rash (butterfly rash), photosensitive reactions, discoid lesions, and oral ulcers common in SLE
- Cardiovascular Complications: Increased risk of myocarditis, pericarditis, atherosclerosis, thrombosis, and valvular disease in anti-dsDNA positive patients
- Pulmonary Involvement: Can cause pleuritis, acute lupus pneumonitis, pulmonary hemorrhage, and chronic interstitial lung disease
- Neurological Complications: Central nervous system involvement including cognitive dysfunction, psychosis, seizures, transverse myelitis, and peripheral neuropathy
- Hematologic Abnormalities: Anti-dsDNA associated with hemolytic anemia, thrombocytopenia, and leukopenia
Follow-up Tests
- Antinuclear Antibody (ANA): Initial screening test for autoimmune disease; nearly all SLE patients are ANA positive
- Anti-Smith (Anti-Sm) Antibodies: Highly specific for SLE; often tested alongside anti-dsDNA for diagnostic confirmation
- Complement Levels (C3, C4): Low complement levels indicate active disease; help monitor disease activity; trend with anti-dsDNA levels
- Complete Blood Count (CBC): Evaluates for cytopenias (anemia, leukopenia, thrombocytopenia) associated with SLE
- Comprehensive Metabolic Panel (CMP): Assesses kidney and liver function; important baseline and monitoring test for SLE patients
- Urinalysis: Screening for proteinuria and hematuria indicating lupus nephritis; correlates with high anti-dsDNA titers
- 24-Hour Urine Protein: Quantifies proteinuria; essential test when lupus nephritis is suspected or confirmed
- Anti-dsDNA Repeat Testing: Performed every 3-6 months in established SLE patients to monitor disease activity; more frequently during suspected flares
- Anti-Histone Antibodies: Elevated in drug-induced lupus; helps differentiate from idiopathic SLE
- Kidney Biopsy: May be necessary in high anti-dsDNA positive patients with proteinuria to confirm lupus nephritis and classify the type for treatment planning
- Imaging Studies: Chest X-ray, echocardiography, and MRI may be indicated based on clinical presentation and anti-dsDNA titers to assess organ involvement
Fasting Required?
- No - Fasting is NOT required for the Anti Ds-DNA test
- Blood Draw Timing: Can be drawn at any time of day; no specific time restriction required
- Patient Preparation: Standard blood draw preparation only; arrive well-hydrated if possible to facilitate blood collection
- Medications: No medications need to be stopped or avoided; continue all regular medications including immunosuppressants and corticosteroids
- Special Instructions: Inform phlebotomist if you have a history of difficult blood draws or have specific arm preferences
- Test Duration: Results typically available within 3-7 business days depending on laboratory processing

How our test process works!