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Culture & Sensitivity, Aerobic bacteria Blood 1 Aerobic, 1-Anerobic(Manual Method)

Bacterial/ Viral
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Report in 168Hrs

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At Home

nofastingrequire

No Fasting Required

Details

Culturing blood to detect bacteria or fungi in the bloodstream

₹1,999₹2,499

20% OFF

Culture & Sensitivity Aerobic bacteria Blood 1 Aerobic1-Anaerobic (Manual Method)

  • Why is it done?
    • Identifies bacterial pathogens causing bacteremia or sepsis by culturing aerobic and anaerobic organisms from blood samples
    • Determines antibiotic sensitivity patterns to guide targeted antimicrobial therapy and prevent treatment failures
    • Ordered when patients present with fever, chills, hypotension, or clinical signs of systemic infection or sepsis
    • Essential for hospitalized patients with suspected bloodstream infections, post-surgical infections, or immunocompromised conditions
    • Manual method provides enhanced detection of fastidious organisms and improved recovery rates compared to automated systems in select cases
    • Typically performed at the time of clinical suspicion of infection, ideally before antibiotic administration
  • Normal Range
    • Negative Result: No growth of bacteria in aerobic or anaerobic culture media after incubation (48-72 hours minimum)
    • Normal Interpretation: Absence of pathogenic organisms indicates no active bacteremia or bloodstream infection at time of collection
    • Units of Measurement: Qualitative result reported as Positive or Negative; when positive, reported with organism identification and colony forming units (CFU) or growth quantification
    • Reference Range Parameters: Negative = 0 CFU/mL; Normal blood cultures should show zero bacterial growth; any growth requires clinical correlation to differentiate true infection from contamination
    • Interpretation Context: Negative results do not exclude infection if drawn after antibiotic initiation; positive results require consideration of clinical presentation, timing of growth, and organism type
  • Interpretation
    • Positive Culture - True Infection: Growth of clinically significant pathogens (e.g., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae) indicates genuine bacteremia requiring urgent treatment
    • Positive Culture - Likely Contaminant: Skin commensals (Coagulase-negative Staphylococcus, Corynebacterium, Bacillus) isolated from single culture or after prolonged incubation may represent contamination; clinical context crucial for differentiation
    • Aerobic vs Anaerobic Findings: Aerobic growth indicates oxygenated environment organisms (Gram-positive cocci, Gram-negative rods); anaerobic growth suggests infection from GI tract, biliary system, or aspiration sources
    • Sensitivity Results Interpretation: Susceptible (S) = organism inhibited by standard antibiotic concentrations; Intermediate (I) = reduced susceptibility; Resistant (R) = organism growth despite antibiotic presence; guides optimal therapy selection
    • Time to Positivity: Early growth (24-48 hours) suggests high-burden bacteremia or virulent organism; delayed growth (>72 hours) may indicate fastidious organisms, low bacterial load, or prior antibiotic exposure
    • Multiple Positive Cultures: Same organism from multiple blood draws indicates true bacteremia; different organisms suggest contamination; repeated isolation of same agent strengthens diagnosis of endocarditis or persistent infection
    • Factors Affecting Results: Prior antibiotic therapy decreases culture positivity; inadequate sample volume reduces sensitivity; improper collection technique increases contamination; immunosuppression may alter organism recovery
  • Associated Organs
    • Primary Organ Systems: Cardiovascular system (heart, blood vessels, endocardium); Immune system (lymphocytes, phagocytes); Central nervous system (in cases of meningitis); Respiratory system (in pneumonia with bacteremia); Genitourinary system (urinary tract sources)
    • Conditions Commonly Associated: Sepsis; Bacteremia; Endocarditis; Meningitis; Osteomyelitis; Pneumonia with systemic spread; Urinary tract infections with systemic involvement; Intra-abdominal infections; Wound infections; Device-related infections (central lines, prosthetic joints)
    • Associated Diseases: Infectious endocarditis; Acute leukemia with sepsis; Immunocompromised states (HIV/AIDS, neutropenia, chemotherapy); Surgical site infections; Healthcare-associated infections; Community-acquired sepsis
    • Potential Complications: Septic shock and multi-organ failure; Disseminated intravascular coagulation; Acute respiratory distress syndrome; Acute kidney injury; Septic arthritis; Brain abscess; Mycotic aneurysm; Death if untreated
    • Organism-Specific Risks: Staphylococcus aureus (including MRSA) - endocarditis, bone/joint infections; Streptococcus pneumoniae - meningitis; Gram-negative rods - endotoxin shock; Anaerobes - polymicrobial infections with poor prognosis
  • Follow-up Tests
    • Recommended if Culture Positive: Repeat blood cultures (48-72 hours apart) to confirm clearance with appropriate therapy and assess for persistent bacteremia
    • Imaging Studies: Echocardiography (transthoracic or transesophageal) for endocarditis evaluation; CT imaging to identify source of infection (abscess, perforation, foreign body)
    • Complementary Laboratory Tests: Complete blood count with differential; Comprehensive metabolic panel (kidney function, liver function); Prothrombin time and activated partial thromboplastin time; Procalcitonin and C-reactive protein (inflammatory markers); Lactate level (prognosis indicator)
    • Source Control Cultures: Cultures from suspected primary site (urine, sputum, cerebrospinal fluid, wound drainage) to identify source infection and confirm organism consistency
    • Monitoring Frequency: Clinical reassessment every 24-48 hours initially; repeat blood cultures until sterilization achieved; follow-up cultures at end of antibiotic therapy for serious infections (endocarditis, osteomyelitis)
    • Specialized Testing: Serum bactericidal level testing (for specific infections); Molecular PCR for fastidious organisms; Extended spectrum beta-lactamase (ESBL) confirmation; Methicillin-resistance confirmation (MRSA); Vancomycin minimum inhibitory concentration (MIC) determination
  • Fasting Required?
    • Fasting: No - Fasting is NOT required for blood culture collection
    • Dietary Restrictions: None - Patient may eat and drink normally; no dietary modifications necessary
    • Medications: Do NOT withhold routine medications; continue all prescribed drugs including antibiotics if already initiated (though pre-antibiotic collection is preferred for better organism isolation)
    • Special Instructions: Collect sample before antibiotic therapy if possible; minimum 10 mL blood volume per culture bottle (5 mL aerobic, 5 mL anaerobic bottles recommended); perform skin antisepsis with chlorhexidine or alcohol prep for 30 seconds before collection
    • Patient Preparation: Collect from different peripheral venipuncture sites (two separate sticks minimum recommended); avoid collection from existing intravenous lines unless specifically indicated; verify patient identification and proper specimen labeling immediately after collection; maintain sterile technique throughout procedure
    • Timing Considerations: Collect during fever spikes when possible but do not delay collection awaiting fever; transport samples to laboratory immediately (within 15 minutes) at room temperature to avoid bacterial overgrowth or death

How our test process works!

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