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Culture & Sensitivity, Aerobic bacteria Clot( Vitek 2 Compact)
Bacterial/ Viral
Report in 72Hrs
At Home
No Fasting Required
Details
Identifies bacteria & antibiotic susceptibility.
₹1,036₹1,480
30% OFF
Culture & Sensitivity Aerobic bacteria Clot (Vitek 2 Compact)
- Why is it done?
- To identify and isolate aerobic bacteria from blood specimens collected in clot tubes (serum separator tubes)
- To determine bacterial species causing bacteremia or sepsis in patients with systemic infections
- To perform antibiotic susceptibility testing using the VITEK 2 Compact system for rapid identification of resistance patterns
- To guide targeted antibiotic therapy selection based on organism susceptibilities
- To monitor and manage patients with confirmed or suspected bloodstream infections, fever of unknown origin, or post-surgical infections
- Typically performed when patients present with signs of systemic infection including fever, chills, hypotension, or sepsis criteria
- Normal Range
- Negative/No Growth: The absence of any bacterial growth after 24-48 hours of incubation indicates no aerobic bacteria present in the blood specimen
- This is the normal/expected result and indicates absence of bacteremia
- Positive/Growth Detected: Identification of bacterial species with colonies growing in culture media
- Results are reported as organism identification (bacterial species name) and qualitative presence rather than numerical values
- Antibiotic Susceptibility Results: Reported as Susceptible (S), Intermediate (I), or Resistant (R) for each antibiotic tested
- Susceptible (S): Organism can be effectively treated with recommended doses of the antibiotic
- Intermediate (I): Organism susceptibility is uncertain; higher than standard doses may be effective
- Resistant (R): Organism is not susceptible to the antibiotic; alternative therapy should be considered
- Interpretation
- No Growth (Negative Result): Indicates absence of aerobic bacteremia; most common finding in healthy individuals or those without bloodstream infection
- Positive Growth - Single Pathogen: Suggests a true bloodstream infection; clinically significant when isolated from appropriate clinical presentation
- Positive Growth - Multiple Organisms: May indicate mixed infection, contamination during collection, or polymicrobial sepsis requiring clinical correlation
- Contaminating Organisms: Skin commensals (Coagulase-negative Staphylococci, Propionibacterium, Corynebacterium) found in single culture may represent contamination, especially if patient lacks symptoms
- Susceptibility Profile Interpretation: Determines which antibiotics are effective for treatment selection
- Multi-Drug Resistant (MDR) Organisms: Resistance to multiple antibiotic classes requires careful therapy selection and infection control measures
- VITEK 2 Compact System: Provides rapid automated identification and susceptibility results within 4-8 hours, enabling faster clinical decision-making compared to traditional methods
- Factors Affecting Results: Improper collection technique, contamination during collection or processing, inadequate sample volume, delayed specimen transport, and use of wrong collection tube type may compromise results
- Prior Antibiotic Therapy: May reduce culture sensitivity or affect susceptibility patterns; collection timing relative to antibiotic initiation is critical
- Associated Organs
- Cardiovascular System: Primary target of bloodstream infections; bacteremia can cause acute endocarditis, septic thrombophlebitis, or septic shock
- Lungs/Respiratory System: Pneumonia and lower respiratory tract infections are common sources of bacteremia
- Urinary Tract/Kidneys: Urinary tract infections and pyelonephritis are major sources of aerobic bacteremia (E. coli, Klebsiella)
- Gastrointestinal Tract: Source of polymicrobial infections with gram-negative and anaerobic organisms
- Central Nervous System: Bacteremia can lead to meningitis or brain abscess formation
- Bones and Joints: Bacteremia can seed bones causing osteomyelitis or septic arthritis
- Liver and Biliary System: Source of aerobic bacterial infections (E. coli, Klebsiella, Enterococcus)
- Skin and Soft Tissues: Can be source of bacteremia from wounds, ulcers, or surgical site infections
- Associated Conditions: Sepsis, septic shock, endocarditis, disseminated infections, osteomyelitis, meningitis, organ dysfunction, and systemic inflammatory response syndrome (SIRS)
- Risk Factors for Bacteremia: Immunosuppression, indwelling catheters, invasive procedures, diabetes mellitus, liver cirrhosis, malignancy, and splenectomy
- Follow-up Tests
- Repeat Blood Cultures: Recommended to document clearance of bacteremia after antibiotic therapy initiation in cases of persistent positive cultures or clinical deterioration
- Source Control Cultures: If primary infection site identified, additional cultures from suspected source (urine, wound, respiratory secretions) help confirm diagnosis
- Complete Blood Count (CBC): To assess white blood cell count and other hematologic parameters in infection
- Inflammatory Markers: C-reactive protein (CRP) and procalcitonin to monitor response to therapy
- Comprehensive Metabolic Panel: To assess organ function (renal, hepatic, metabolic abnormalities) in sepsis
- Lactate Level: Prognostic marker of tissue perfusion and severity of sepsis
- Coagulation Studies: PT/INR and aPTT to assess for disseminated intravascular coagulation (DIC) in severe sepsis
- Imaging Studies: Chest X-ray, CT abdomen/pelvis, or other imaging to identify source of infection
- Echocardiography: If endocarditis suspected based on organism type or clinical presentation
- Extended Resistance Testing: Additional testing for specific resistance mechanisms (ESBL, carbapenemase) if clinically indicated
- Continued Monitoring: Serial cultures, lab work, and clinical assessment during antimicrobial therapy course to assess treatment response
- Fasting Required?
- No - Fasting is NOT required for this test. Blood can be collected from patients at any time regardless of meal status or time of day.
- Collection Timing: Blood cultures should ideally be collected before antibiotic therapy initiation or as soon as sepsis is suspected to maximize culture sensitivity
- Specimen Collection Requirements: Use sterile serum separator tubes (SST/clot tubes) as specified by laboratory protocol
- Aseptic Technique: Proper skin antisepsis (70% alcohol or chlorhexidine) is essential to minimize contamination risk; cleanse collection site for at least 30 seconds
- Multiple Cultures: Two or more sets from different venipuncture sites are recommended for optimal culture sensitivity; allows differentiation of contamination from true bacteremia
- Sample Volume: Adequate blood volume (typically 8-10 mL per culture set per laboratory specifications) is critical for optimal organism recovery
- Transport Conditions: Specimens should be transported to laboratory promptly at room temperature without refrigeration; do not delay transport
- Medications: No specific medications need to be held; however, antibiotic initiation should not significantly delay specimen collection
- Patient Preparation: No special preparation required; routine venipuncture safety precautions apply
How our test process works!
