Culture & Sensitivity, Aerobic bacteria Pus(Vitek 2 Compact)

Bacterial/ Viral

Report in 72Hrs

At Home

No Fasting Required

Details

Identifies bacteria & antibiotic susceptibility.

₹1,036₹1,480

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Culture & Sensitivity Aerobic bacteria Pus(Vitek 2 Compact) - Comprehensive Test Guide

Section 1: Why is it done?
- Purpose: This test identifies and isolates aerobic bacteria from pus samples using the Vitek 2 Compact automated system, which provides rapid organism identification and antimicrobial susceptibility results to guide targeted antibiotic therapy.
- Primary Indications: Suspected bacterial infection with purulent discharge; diagnosis of localized infections; determination of appropriate antibiotic therapy; monitoring treatment response in pyogenic infections
- Common Clinical Scenarios: Abscess drainage; wound infections; skin and soft tissue infections; osteomyelitis; diabetic foot ulcers; post-surgical infections; drainage from boils or cysts
- Timing: Performed when infection is suspected; typically collected at initial presentation of suspected bacterial infection; results available in 24-48 hours using Vitek 2 Compact system
Section 2: Normal Range
- Normal Result: No growth or negative culture - indicates absence of aerobic bacterial infection in the pus sample
- Interpretation of Negative Results: May indicate adequate antibiotic therapy, anaerobic infection, fungal infection, viral infection, or contaminated specimen; clinical correlation with symptoms is essential
- Abnormal Result: Growth of aerobic bacteria - indicates bacterial infection requiring targeted antimicrobial therapy
- Colony Quantification: Results reported as colony-forming units per milliliter (CFU/mL) or light/moderate/heavy growth; ≥1000 CFU/mL typically considered significant for pus samples
- Units of Measurement: Colony-forming units per milliliter (CFU/mL); organism identification by species name; susceptibility reported as Susceptible (S), Intermediate (I), or Resistant (R)
Section 3: Interpretation
- Positive Culture Results: Indicates active aerobic bacterial infection; organism identification guides targeted therapy; heavy growth suggests acute infection; light growth may indicate contamination or previous antibiotic exposure
- Antimicrobial Susceptibility Interpretation: Susceptible (S) = organism inhibited by standard antibiotic doses; Intermediate (I) = partial inhibition, high doses may be effective; Resistant (R) = organism not inhibited by standard doses; results guide appropriate antibiotic selection
- Common Aerobic Pathogens: Staphylococcus aureus (including MRSA); Streptococcus species; Escherichia coli; Pseudomonas aeruginosa; Klebsiella pneumoniae; Proteus species; Acinetobacter species; Enterococcus species
- Clinical Significance of Results: Confirms bacterial infection and identifies causative organism; enables de-escalation or escalation of antibiotic therapy; helps prevent unnecessary broad-spectrum antibiotic use; guides infection control measures; identifies multi-drug resistant organisms requiring special management
- Factors Affecting Results: Prior antibiotic therapy may reduce isolation rates; improper specimen collection or transport affects results; contamination during collection may yield non-pathogenic organisms; mixed cultures indicate polymicrobial infection; sample quality is critical for accurate identification
- Vitek 2 Compact System Advantages: Rapid automated identification and susceptibility testing within 24-48 hours; standardized methodology reduces operator error; provides quantitative susceptibility minimum inhibitory concentrations (MIC); enables rapid therapeutic decision-making
Section 4: Associated Organs
- Anatomical Sites of Infection: Skin and subcutaneous tissues (abscesses, cellulitis, impetigo); bone and joints (osteomyelitis, septic arthritis); internal organs (liver abscess, lung abscess, perirectal abscess); wounds and surgical sites; urinary tract; respiratory tract; gastrointestinal tract
- Primary Organ Systems Involved: Integumentary system (skin infections); musculoskeletal system (bone and joint infections); central nervous system (brain abscess); respiratory system (lung abscess, empyema); gastrointestinal system (intra-abdominal abscess); hepatic system; genitourinary system
- Common Conditions Diagnosed: Bacterial abscess; cellulitis; impetigo; osteomyelitis; septic arthritis; diabetic foot infection; surgical site infection; post-traumatic infection; infected sebaceous cyst; pilonidal disease infection; acne conglobata; infected burn wounds
- Potential Complications of Untreated Infection: Bacteremia and sepsis; septic shock; endocarditis; meningitis; osteomyelitis progression; systemic inflammatory response syndrome (SIRS); multiorgan failure; tissue necrosis; permanent scarring or disfigurement; loss of limb function; death if untreated
- Risk Factors for Severe Infection: Diabetes mellitus; immunosuppression; advanced age; chronic kidney disease; poor nutritional status; obesity; vascular disease; recent surgery; trauma; insertion of foreign bodies
Section 5: Follow-up Tests
- Recommended Follow-up Tests: Repeat culture if clinical deterioration occurs despite appropriate therapy; blood cultures if sepsis is suspected; imaging studies (ultrasound, CT, MRI) to assess extent of infection; anaerobic culture if mixed infection suspected; fungal culture if immunocompromised
- Complementary Tests: Complete blood count (CBC) to assess white blood cell response; C-reactive protein (CRP) for inflammation markers; erythrocyte sedimentation rate (ESR); procalcitonin for severe infection; prothrombin time (PT) and partial thromboplastin time (PTT) if coagulopathy suspected; lactate levels for tissue perfusion assessment
- Monitoring During Treatment: Clinical assessment of wound healing; repeat cultures 48-72 hours after initiation of appropriate therapy; imaging studies 1-2 weeks post-treatment to confirm resolution; follow-up cultures if infection persists beyond expected timeframe
- Extended Antibiotic Susceptibility Tests: E-test or broth microdilution for specific organisms with borderline susceptibility; MRSA confirmation testing; vancomycin minimum inhibitory concentration (MIC) determination for resistant strains; carbapenemase testing for resistant Gram-negative organisms
- Monitoring Frequency: Initial assessment within 24-48 hours; if no improvement, reassess and repeat culture; routine monitoring at 1 week post-treatment; extended monitoring for chronic infections or immunocompromised patients; long-term follow-up for osteomyelitis (weeks to months)
Section 6: Fasting Required?
- Fasting Required: No - Fasting is NOT required for pus culture and sensitivity testing
- Pre-Collection Preparation: Patient should not eat or drink from the site of collection for at least 30 minutes prior to sampling; maintain normal hygiene practices; do not apply topical antiseptics or antibiotic creams immediately before collection unless specified by provider
- Specimen Collection Guidelines: Collect pus directly from abscess or wound using sterile technique; use sterile swab, needle aspiration, or sterile container; avoid surface contaminants or normal flora; collect adequate volume (minimum 1 mL pus recommended); place in sterile container or culture tube
- Medications to Avoid: Do not apply topical antibiotics at collection site for at least 24 hours prior to collection; avoid topical antiseptics (betadine, chlorhexidine) at collection site immediately before sample collection; systemic antibiotics do not require discontinuation for specimen collection
- Post-Collection Instructions: Transport specimen to laboratory immediately; if delay is anticipated, keep specimen at room temperature (not refrigerated); label specimen clearly with patient identifiers and source; do not freeze specimen unless specifically instructed; maintain specimen sterility during transport
- General Patient Instructions: Attend collection appointment as scheduled; inform healthcare provider of all medications being taken; report if currently on antibiotic therapy; inform provider of immunocompromised status; no special physical restrictions required after sample collection

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