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Culture & Sensitivity, Aerobic bacteria Tissue /Biopsy(Vitek 2 Compact)

Biopsy
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Report in 72Hrs

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No Fasting Required

Details

Identifies bacteria & antibiotic susceptibility.

₹1,628₹2,326

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Culture & Sensitivity Aerobic bacteria Tissue/Biopsy (Vitek 2 Compact)

  • Why is it done?
    • Identifies and isolates aerobic bacterial organisms present in tissue or biopsy specimens from infected or potentially infected sites
    • Determines antimicrobial susceptibility patterns using the Vitek 2 Compact automated system for rapid and accurate identification
    • Guides appropriate targeted antibiotic therapy selection and prevents unnecessary broad-spectrum antimicrobial use
    • Ordered when tissue or biopsy specimens show signs of infection, inflammation, or when bacterial infection is clinically suspected
    • Performed on specimens from wounds, abscesses, surgical sites, deep tissue biopsies, bone, joint fluids, and other infected tissues
    • Typically performed within 24-72 hours of specimen collection to ensure optimal bacterial viability and recovery
  • Normal Range
    • Normal Result: No growth or negative culture - indicates absence of aerobic bacterial pathogens in the tissue/biopsy specimen
    • Growth Present: Abnormal result - indicates bacterial infection; results reported as organism identification with organism name and category (e.g., Staphylococcus aureus)
    • Quantification: Results may be reported as colony forming units (CFU) or as rare, moderate, or heavy growth to indicate bacterial load
    • Sensitivity Interpretation: Susceptible (S) = organism inhibited by standard antibiotic concentrations; Resistant (R) = organism not inhibited; Intermediate (I) = organism moderately inhibited
    • Units: Results provided as organism identification with specific antibiotic susceptibility categories (S/I/R) for each tested antimicrobial agent
  • Interpretation
    • No Growth/Negative Culture: Suggests absence of aerobic bacterial infection or may indicate improper specimen collection, delayed processing, or presence of only anaerobic organisms requiring different culture methods
    • Single Organism Growth (Pathogen): Typically clinically significant, representing true infection; organism identification guides specific antibiotic selection
    • Multiple Organisms Growth: May indicate polymicrobial infection or possible contamination; clinical correlation necessary; commensal organisms may represent normal flora or skin contaminants
    • Susceptible (S) Results: Organism should respond to standard therapeutic doses of the antibiotic; first-line therapy choice; lowest minimum inhibitory concentration (MIC) values
    • Intermediate (I) Results: Organism may respond to higher antibiotic doses; uncertain clinical outcome; consider alternative agents or use only if no susceptible options available or specific site concentrations achieve therapeutic levels
    • Resistant (R) Results: Organism unlikely to respond to standard therapeutic doses; alternative antibiotic necessary; higher MIC values; avoid this antibiotic unless specific clinical circumstances warrant
    • Vitek 2 Compact System Advantages: Provides rapid automated identification (typically 4-8 hours) and susceptibility testing; reduces time to appropriate therapy; utilizes advanced biochemical and growth-based methodology for accurate organism identification
    • Factors Affecting Results: Prior antibiotic therapy may reduce organism recovery; specimen contamination affects interpretation; specimen adequacy and collection method impact results; delay in processing reduces bacterial viability
  • Associated Organs
    • Primary Organ Systems Involved: Integumentary system (skin and soft tissues); respiratory system; gastrointestinal system; musculoskeletal system (bone and joints); urogenital system; central and peripheral nervous system
    • Common Conditions Associated with Positive Results: Surgical wound infections; diabetic foot ulcers; abscesses; osteomyelitis (bone infection); septic arthritis; necrotizing fasciitis; cellulitis; chronic wound infections; prosthetic joint infections; post-operative complications
    • Typical Bacterial Isolates: Staphylococcus aureus (including MRSA); Streptococcus species; Enterococcus species; E. coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Proteus species; Acinetobacter; anaerobic species (if aerobic culture positive, anaerobic may also be ordered)
    • Medical Conditions Diagnosed or Monitored: Bacterial infections of various tissue types; nosocomial (hospital-acquired) infections; healthcare-associated infections; antibiotic resistance patterns; treatment failure investigation; immunocompromised patient infections
    • Potential Complications from Untreated Positive Results: Sepsis and septic shock; bacteremia and systemic infection; chronic osteomyelitis; tissue necrosis and loss; amputations (in diabetic foot infections); delayed wound healing; treatment complications; metastatic infections to remote sites; death if untreated
    • Risk Factors for Positive Results: Diabetes mellitus; immunosuppression; recent surgery; vascular insufficiency; obesity; poor nutrition; advanced age; chronic wounds; prosthetic implants; invasive procedures; hospitalization
  • Follow-up Tests
    • If Positive Culture Results: Repeat tissue culture to confirm organism if clinical response inadequate; anaerobic culture if polymicrobial infection suspected; fungal culture if immunocompromised; blood cultures if systemic infection suspected
    • Microbiological Follow-up Tests: Extended susceptibility panels for resistant organisms; ESBL (Extended-Spectrum Beta-Lactamase) detection; MRSA confirmation; beta-lactamase production testing; vancomycin-resistant enterococci (VRE) testing
    • Clinical Laboratory Tests: Complete blood count (CBC); C-reactive protein (CRP); procalcitonin; serum lactate; blood glucose; inflammatory markers; differential white blood cell count; albumin levels
    • Imaging Studies: Ultrasound to assess soft tissue extent; CT scan for deep tissue involvement; MRI for bone/osteomyelitis assessment; X-ray for bone infection evaluation; nuclear medicine bone scans
    • Treatment Response Monitoring: Repeat tissue culture after 7-14 days of therapy to document sterilization; repeat biopsy if clinical deterioration occurs; surveillance cultures post-antibiotic therapy; repeat imaging at treatment intervals
    • Related Complementary Tests: Gram stain and microscopy; aerobic blood cultures; urine culture; sputum culture; wound swab culture; abscess fluid culture depending on infection site; PCR for rapid organism identification
    • Monitoring Frequency for Ongoing Conditions: For chronic infections: repeat cultures every 1-2 weeks initially; monthly once stabilized; for osteomyelitis: cultures at 4-6 weeks after antibiotic initiation; for surgical site infections: repeat cultures if fever persists or drainage increases
    • If Negative or No Growth: Repeat culture if clinical suspicion remains high; anaerobic culture if not already performed; fungal culture if immunocompromised or empiric antifungal therapy initiated; serology for fastidious organisms
  • Fasting Required?
    • Fasting Required: No - This is a tissue/biopsy specimen test; fasting is not required
    • Specimen Collection Requirements: Tissue or biopsy sample collected using sterile technique during biopsy procedure or surgical intervention; specimen placed in sterile container; minimal disinfectant use on collection site to avoid contamination
    • Specimen Handling Instructions: Transport specimen to laboratory immediately within 1-2 hours; keep at room temperature unless anaerobic culture also ordered; do not refrigerate tissue specimens as this may kill fastidious organisms; use sterile swabs or containers only
    • Medications to Avoid: If possible, delay antibiotics until after tissue specimen collection; if antibiotics already initiated, document timing and specific agents as this affects culture results; topical antiseptics should not be applied to collection site immediately before biopsy
    • Pre-Procedure Preparation: Informed consent for biopsy procedure; NPO (nothing by mouth) status if procedure requires anesthesia (per anesthesia requirements); patient positioning as needed for tissue access; local or regional anesthesia as appropriate
    • Specimen Labeling and Documentation: Clearly label specimen with patient name, ID, date and time of collection; document specimen source location (e.g., left knee abscess); note any prior antibiotics; document collection method; specify if anaerobic culture also needed
    • Post-Specimen Collection Care: Observe for bleeding or other complications at biopsy site; maintain sterile dressing; monitor for infection signs; report fever, excessive drainage, or increasing pain; follow wound care instructions provided by proceduralist

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