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Free Testosterone

Unit Test
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Report in 24Hrs

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At Home

fastingrequire

Fasting Required

Details

Measures total testosterone for assessment of male hypogonadism, PCOS, and infertility.

4991485

66% OFF

🧪 What is Free Testosterone?

Free testosterone refers to the biologically active portion of testosterone that is not bound to proteins in the blood.

  • Total testosterone includes:
    • Free testosterone (~2%)
    • Testosterone bound to SHBG (sex hormone-binding globulin) (~65%)
    • Testosterone bound to albumin (~33%, weakly bound)

Only free testosterone is considered bioavailable and able to exert physiological effects on target tissues.

❓ Why is the Free Testosterone Test Done?

To:

  • Evaluate hypogonadism (testosterone deficiency) in men
  • Investigate infertility, erectile dysfunction, decreased libido
  • Assess delayed or precocious puberty
  • Diagnose polycystic ovarian syndrome (PCOS) or hirsutism in women
  • Differentiate between true low testosterone and low total testosterone with normal free testosterone (e.g., due to high SHBG)

📊 Normal Ranges

Group

Normal Free Testosterone Range

Men (Adult)

5 – 21 ng/dL (or 174 – 729 pmol/L)

Women (Adult)

0.3 – 1.9 ng/dL (or 10 – 66 pmol/L)

Children / Adolescents

Age-dependent; lower than adults

🔍 Values can vary by age, lab method, and SHBG levels.
Calculated free testosterone is sometimes used instead of direct measurement (via total T + SHBG + albumin).

📈 Interpretation of Results

Level

Interpretation

🔻 Low Free Testosterone

- Primary hypogonadism (testicular failure)
- Secondary hypogonadism (pituitary/hypothalamic dysfunction)
- Aging or obesity
- Chronic illness, opioid use, or liver disease

🔺 High Free Testosterone

- PCOS, adrenal or ovarian tumors (in women)
- Anabolic steroid use (in men)
- Congenital adrenal hyperplasia (CAH)
- Rare testosterone-secreting tumors

🧠 Associated Organs and Conditions

Organ/System

Role

Testes (Leydig cells)

Produce testosterone in males

Ovaries & Adrenals

Contribute to testosterone in females

Pituitary gland

Regulates testosterone via LH secretion

Liver

Produces SHBG, affects free vs bound testosterone balance

Hypothalamus

Controls GnRH → LH/FSH → Testosterone

🔄 Related / Follow-Up Tests

  1. Total Testosterone
  2. SHBG (Sex Hormone-Binding Globulin)
  3. Albumin – Used for calculated free T
  4. LH and FSH – Evaluate pituitary function
  5. Estradiol – Especially in men with gynecomastia
  6. DHEA-S / Androstenedione – In hirsutism or virilization
  7. Prolactin – Elevated in secondary hypogonadism
  8. MRI Pituitary – If central cause suspected

✅ Fasting Required?

Parameter

Requirement

Fasting Required?

✅ YesMorning sample (7–10 AM) and 8–10 hours fasting recommended, as testosterone levels are highest in the morning and fluctuate throughout the day.

📝 Summary

Parameter

Summary

What

Measures the biologically active, unbound testosterone available in circulation

Why Test

To evaluate hypogonadism, infertility, PCOS, or pubertal disorders

Normal Range

Men: 5–21 ng/dL; Women: 0.3–1.9 ng/dL

Low Levels

Suggest testosterone deficiency, aging, pituitary or testicular dysfunction

High Levels

Seen in PCOS, tumors, steroid use

Follow-up Tests

Total T, SHBG, LH, FSH, DHEA-S, Prolactin, Estradiol

Fasting Required

✅ Yes (morning sample preferred)

How our test process works!

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