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Fungal Stain (KOH Mount)

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KOH mount (Potassium Hydroxide Mount) is a simple, rapid microscopic test used to detect fungal elements like hyphae, spores, or yeast cells

99150

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🧪 What is Fungal Stain (KOH Mount)?

KOH mount (Potassium Hydroxide Mount) is a simple, rapid microscopic test used to detect fungal elements like hyphae, spores, or yeast cells in clinical samples.

  • 10–20% KOH solution is added to skin scrapings, nail clippings, hair shafts, or body fluids.
  • KOH dissolves keratin and cellular debris, making fungal structures more visible under a microscope.
  • It's a screening tool, not a confirmatory test — does not identify the fungal species.

❓ Why is the KOH Mount Done?

To:

  • Quickly detect superficial fungal infections (e.g., skin, nails, hair)
  • Screen vaginal, oral, or corneal fungal infections
  • Evaluate persistent itching, scaling, or discoloration
  • Distinguish fungal from bacterial or non-infective skin conditions

🔍 Common Specimen Types

  • Skin scrapings – from lesions, rashes, or scaling areas
  • Nail clippings – in suspected onychomycosis
  • Hair shafts – in scalp fungal infections
  • Vaginal swabs, oral scrapings, sputum, CSF, corneal scrapings

📊 Normal/Abnormal Findings

Result

Interpretation

No fungal elements seen

Normal – No evidence of fungal infection

Fungal elements seen

Abnormal – Presence of hyphae, pseudohyphae, or yeast cells suggests fungal infection

May be followed by fungal culture for species identification.

📈 Interpretation of Results

Finding

Suggestive of

Septate hyphae

Dermatophytes (e.g., Trichophyton, Microsporum)

Non-septate hyphae

Zygomycosis (Mucor, Rhizopus)

Budding yeast cells

Candida spp.

Pseudohyphae + budding

Candida albicans

Spherical yeast cells with capsule

Cryptococcus (India ink may also be used)

🧠 Associated Organs and Conditions

Site

Common Fungal Conditions

Skin

Tinea corporis, Tinea cruris (ringworm)

Nails

Onychomycosis

Hair/scalp

Tinea capitis

Vagina

Candidiasis

Mouth

Oral thrush

Cornea

Fungal keratitis

CSF

Cryptococcal meningitis (with India ink/KOH)

🔄 Related / Follow-Up Tests

  1. Fungal Culture – For definitive species identification
  2. Gram Stain / PAS / GMS stain – For deeper fungal structures
  3. KOH + Calcofluor White stain – Enhances visibility under fluorescence
  4. Histopathology – For invasive fungal infections
  5. Serologic tests – e.g., cryptococcal antigen, galactomannan
  6. PCR or molecular panels – For systemic fungal infections

✅ Fasting Required?

Parameter

Requirement

KOH Mount

❌ No fasting required – Sample is collected directly from affected site, irrespective of food intake

📝 Summary

Parameter

Summary

What

Microscopic screening test using KOH to detect fungal structures in skin, nails, hair, or fluids

Why Test

Rapid detection of fungal infections, especially superficial mycoses

Result Interpretation

Hyphae/yeast = Positive; No elements = Negative

Common Conditions

Tinea, candidiasis, onychomycosis, fungal keratitis

Follow-up Tests

Fungal culture, special stains, PCR, serology

Fasting Required

❌ No

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