jamunjar-logo
whatsapp
cartmembermenu
Search for
"test & packages"
"physiotherapy"
"heart"
"lungs"
"diabetes"
"kidney"
"liver"
"cancer"
"thyroid"
"bones"
"fever"
"vitamin"
"iron"
"HTN"

Neisseria Gonorrhoea DNA Detection by Real Time PCR

Reproductive
image

Report in 72Hrs

image

At Home

nofastingrequire

No Fasting Required

Details

Detects gonorrhea infection.

₹4,440₹6,343

30% OFF

Neisseria Gonorrhoea DNA Detection by Real Time PCR

  • Why is it done?
    • Test Description: This test detects the presence of Neisseria gonorrhoea DNA using real-time polymerase chain reaction (PCR), a highly sensitive molecular technique that amplifies and identifies pathogen-specific genetic material
    • Primary Indications: Diagnosis of active gonorrhea infection in symptomatic patients presenting with urethritis, cervicitis, proctitis, or pharyngitis
    • Screening Purposes: Routine screening in asymptomatic individuals, pregnant women, and those with multiple sexual partners or known exposure to infected partners
    • Post-Treatment Monitoring: Test of cure performed 3-5 weeks after completion of antibiotic therapy to confirm eradication of infection
    • Contact Tracing: Testing of sexual partners of confirmed gonorrhea cases for epidemiological control and prevention of transmission
    • Specimen Types Tested: First-void urine, urethral swabs, endocervical swabs, rectal swabs, pharyngeal swabs, or vaginal swabs depending on clinical presentation and site of suspected infection
  • Normal Range
    • Result Format: Qualitative (not quantitative) - reported as either DETECTED or NOT DETECTED
    • Normal/Negative Result: NOT DETECTED - indicates absence of Neisseria gonorrhoea DNA in the specimen; patient is not infected with gonorrhea
    • Abnormal/Positive Result: DETECTED - indicates presence of Neisseria gonorrhoea DNA; patient has active or recent gonorrhea infection
    • Units of Measurement: No numerical value reported; PCR threshold cycle (Ct) values are used internally for detection but typically not provided in clinical reports
    • Sensitivity and Specificity: Real-time PCR demonstrates >95% sensitivity and >99% specificity compared to culture and other molecular methods, making it the gold standard for gonorrhea diagnosis
    • Interpretation Guide: A negative result in a symptomatic patient does not completely exclude gonorrhea if the test was performed during early infection before adequate pathogen shedding, or if an improper specimen collection technique was used
  • Interpretation
    • Positive Result (DETECTED): Confirms active Neisseria gonorrhoea infection requiring immediate antibiotic treatment, typically with cephalosporin-based regimens (ceftriaxone 500 mg IM single dose or azithromycin 1g orally); sexual partners must be notified and tested; CDC reporting requirements must be met
    • Negative Result (NOT DETECTED): Rules out gonorrhea infection in most cases; however, very early infection (first few days) may show negative results as bacterial DNA may not be present in sufficient quantities for detection
    • Factors Affecting Results: Improper specimen collection or transport, contamination, timing of specimen collection relative to infection onset, presence of inhibitors in specimen, antibiotic use prior to testing (may result in false negatives), and concurrent urinary tract infections may affect test accuracy
    • Clinical Significance of Results: Positive results indicate acute gonorrhea requiring urgent treatment to prevent complications such as pelvic inflammatory disease (PID), epididymitis, infertility, and disseminated gonococcal infection (DGI); results also have public health implications requiring notification of sexual contacts and surveillance reporting
    • Result Patterns and Interpretation: Single positive result confirms infection; persistently negative results in symptomatic patients suggest alternative diagnoses (non-gonococcal urethritis, other STIs, urinary tract infection); positive results post-treatment indicate treatment failure or reinfection requiring additional culture and susceptibility testing for antimicrobial resistance evaluation
    • Dual Infection Considerations: Gonorrhea often occurs concurrently with Chlamydia trachomatis (30-50% of cases); simultaneous testing for both pathogens using multiplexed PCR is recommended; positive gonorrhea results should always prompt testing for concurrent chlamydial infection
  • Associated Organs
    • Primary Organ Systems: Urogenital tract (urethra, bladder), reproductive system (prostate, seminal vesicles in males; endocervix, uterus, fallopian tubes in females), rectum, and pharynx
    • Associated Medical Conditions: Acute urethritis (males), acute cervicitis (females), proctitis, pharyngitis, pelvic inflammatory disease (PID), endometritis, salpingitis, prostatitis, epididymitis, orchitis, bartholinitis, and urinary tract infections
    • Systemic Complications: Disseminated gonococcal infection (DGI) with septic arthritis, tenosynovitis, skin lesions, and meningitis in untreated cases; vertical transmission to neonates causing ophthalmic neonatorum (gonococcal conjunctivitis)
    • Long-term Complications: Infertility due to fallopian tube scarring (females), ectopic pregnancy, chronic pelvic pain, urethral strictures (males), and increased susceptibility to other sexually transmitted infections including HIV
    • Complications in Pregnancy: Increased risk of preterm delivery, premature rupture of membranes, spontaneous abortion, and vertical transmission to neonate at delivery with potential for gonococcal ophthalmia neonatorum and disseminated neonatal infection
    • Risk Factors for Severe Outcomes: Delayed diagnosis and treatment, immunocompromised status, pregnancy, presence of other STIs, antimicrobial resistance, and failure to treat sexual partners
  • Follow-up Tests
    • Chlamydia trachomatis Testing: Mandatory concurrent or simultaneous testing recommended due to high rate of coinfection (30-50%); use of multiplexed PCR assay allows detection of both pathogens from single specimen
    • Test of Cure: Repeat PCR testing 3-5 weeks after completion of antibiotic therapy to confirm eradication; not recommended earlier than 3 weeks due to risk of false positive results from residual DNA; recommended especially in areas with high antimicrobial resistance rates or suspected treatment failure
    • Culture and Antimicrobial Susceptibility Testing: Recommended if PCR is positive to obtain culture for susceptibility testing, particularly in cases of treatment failure, antibiotic allergy requiring alternative therapy, or concerns about emerging resistance patterns; culture also allows identification of organism viability
    • Other Sexually Transmitted Infection (STI) Panel: Testing for HIV, syphilis (RPR/VDRL and FTA-ABS confirmatory testing), HSV-1/HSV-2, and hepatitis B should be performed at time of gonorrhea diagnosis or within designated follow-up period as per CDC guidelines
    • Pregnancy-Related Testing: Repeat gonorrhea and chlamydia PCR testing in third trimester (28-32 weeks) for pregnant women diagnosed in first or second trimester; cultures should be obtained to guide therapy decisions and identify resistant organisms in pregnancy
    • Partner Testing and Contact Tracing: Testing of all sexual partners within 60 days prior to symptom onset recommended; evaluation and empiric treatment of partners regardless of test results when epidemiologically linked to confirmed gonorrhea case
    • Neonatal Screening: Infants born to mothers with untreated gonorrhea require prophylactic treatment and close follow-up; cultures from conjunctival discharge if neonatal conjunctivitis develops
    • Monitoring Frequency: All patients should receive annual rescreening as part of routine STI prevention strategy, particularly those with multiple partners or previous gonorrhea infections; pregnant women should be screened at first prenatal visit and again in third trimester
  • Fasting Required?
    • Fasting Requirement: NO - Fasting is not required for this test
    • Specimen Collection Preparation: For urine specimens: patient should not urinate for 1-2 hours prior to collection; use first-void morning urine or first-catch urine for optimal bacterial shedding
    • Pre-Test Restrictions: Patient should avoid urination for 1-2 hours before specimen collection; avoid douching (females) for 24 hours prior to cervical swab collection; avoid rectal douching before rectal specimen collection; avoid mouthwash before pharyngeal specimen collection
    • Medication Considerations: No medications need to be withheld prior to testing; however, antibiotic use within 7 days prior to specimen collection may result in false negative results and should be documented; urinary antiseptics and lubricants containing bacteriostatic agents should be avoided
    • General Patient Instructions: Avoid sexual intercourse for 24 hours prior to specimen collection; abstain from alcohol use for 48 hours prior if possible; maintain good hygiene but do not clean urethral area immediately before urine collection; inform healthcare provider of recent antibiotic use
    • Special Collection Instructions: Use proper sterile collection tubes with appropriate transport media; swabs must contact mucosal surfaces directly; specimens must be transported to laboratory promptly (within 2 hours) or refrigerated if delayed to maintain organism viability and DNA integrity; document specimen source, collection time, and patient symptoms at time of collection

How our test process works!

customers
customers