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Vitamin B2 (Riboflavin) - Quantitative, EDTA Blood
Vitamin
Report in 192Hrs
At Home
Fasting Required
Details
Measures vitamin levels.
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Vitamin B2 (Riboflavin) - Quantitative EDTA Blood
- Why is it done?
- Test Purpose: Measures the concentration of vitamin B2 (riboflavin) in blood using EDTA anticoagulant tube. This test assesses nutritional status and identifies deficiency states that may cause clinical symptoms.
- Primary Indications: Evaluation of suspected riboflavin deficiency, investigation of peripheral neuropathy, assessment of nutritional status in malabsorption syndromes, evaluation of dermatological symptoms (angular cheilitis, seborrheic dermatitis), investigation of photophobia or corneal abnormalities, monitoring in patients with chronic alcoholism, assessment of migraine frequency.
- Clinical Scenarios: Patients with inflammatory bowel disease, celiac disease, or other malabsorption disorders; individuals with restricted dietary intake or strict vegan diets; patients on certain medications affecting riboflavin absorption; assessment during nutritional rehabilitation programs; evaluation of fatigue or weakness of unclear etiology.
- Normal Range
- Reference Values: Normal riboflavin levels typically range from 4.0-24 µg/L (micrograms per liter) or 1.5-9.0 nmol/L (nanomoles per liter), though reference ranges may vary slightly between laboratories. Units of measurement are typically expressed as µg/dL (micrograms per deciliter) or nmol/L.
- Result Interpretation: LOW (<4 µg/L): Riboflavin deficiency present, suggests inadequate intake or absorption; BORDERLINE (4-8 µg/L): Marginal deficiency, potential biochemical deficiency; NORMAL (8-24 µg/L): Adequate riboflavin stores; HIGH (>24 µg/L): Excessive supplementation, generally non-toxic due to urinary excretion of water-soluble vitamin.
- Normal vs. Abnormal: Normal results indicate sufficient riboflavin status for metabolic function. Low levels indicate deficiency requiring dietary intervention or supplementation. Results must be interpreted in clinical context with patient symptoms and dietary history, as deficiency develops progressively and tissue depletion precedes clinical manifestations.
- Interpretation
- Severe Deficiency (<2 µg/L): Indicates clinical riboflavin deficiency (ariboflavinosis). Patient likely experiencing symptoms including angular cheilitis, glossitis, seborrheic dermatitis, photophobia, and potential ocular complications. Requires immediate therapeutic intervention.
- Mild-to-Moderate Deficiency (2-4 µg/L): Indicates inadequate riboflavin stores. May present with subtle symptoms including fatigue, weakness, peripheral neuropathy, or mild skin changes. Increased susceptibility to infections due to impaired immune function. Requires dietary modification and supplementation.
- Low-Normal (4-8 µg/L): Borderline status indicating marginal deficiency. Patient may be at risk for developing clinical symptoms with further depletion. Recommend dietary counseling and consideration of supplementation in high-risk populations.
- Normal (8-24 µg/L): Adequate riboflavin status. Sufficient stores to maintain normal metabolic function, energy production, and cellular antioxidant defense mechanisms.
- Elevated (>24 µg/L): Indicates excessive supplementation or parenteral administration. Generally not harmful since riboflavin is water-soluble and excreted via urine. Suggests unnecessary supplementation or recent high-dose intake.
- Factors Affecting Results: Recent dietary intake significantly influences levels; supplementation, medications (phenothiazines, tricyclic antidepressants), malabsorption diseases, liver dysfunction, chronic alcoholism, dialysis, and recent transfusions can affect values. Hemolysis may interfere with results. Season may affect levels due to dietary variations.
- Associated Organs
- Primary Organ Systems: Gastrointestinal tract (absorption site), liver (storage and metabolism), nervous system (myelin formation and neural function), skin (structural integrity and repair), eyes (photoreceptor function), mitochondria (energy production).
- Associated Conditions with Deficiency: Celiac disease, Crohn's disease, ulcerative colitis, tropical sprue, short bowel syndrome, chronic diarrhea, cystic fibrosis, liver cirrhosis, chronic alcoholism, dialysis-dependent renal failure, hyperthyroidism, hypermetabolic states, cancer cachexia.
- Clinical Manifestations of Deficiency: Angular cheilitis (cracks at mouth corners), glossitis (swollen tongue), seborrheic dermatitis affecting face and trunk, keratitis and photophobia (eye involvement), vertical ridges in nails, peripheral neuropathy with paresthesias, fatigue, generalized weakness, growth retardation in children.
- Potential Complications: Progressive neurological damage if untreated, vision impairment or corneal scarring, severe dermatological involvement affecting quality of life, secondary infections due to impaired skin integrity, increased frequency of migraines, progression to severe neuropathy causing disability.
- Follow-up Tests
- Recommended Additional Tests: Other B-complex vitamins (B1, B3, B6, B12, folate) to evaluate for concurrent deficiencies; complete metabolic panel to assess liver and kidney function; complete blood count for macrocytic anemia; tissue transglutaminase (tTG) or endomysial antibodies if celiac disease suspected; albumin and prealbumin for overall nutritional assessment.
- Complementary Diagnostic Tests: Erythrocyte glutathione reductase activity coefficient (EGRAC) - functional marker of riboflavin status; flavin adenine dinucleotide (FAD) levels; assessment of malabsorption including 72-hour stool fat, lactose tolerance testing if indicated.
- Imaging and Specialty Studies: Upper endoscopy or colonoscopy if inflammatory bowel disease suspected; ophthalmologic evaluation if ocular symptoms present; electromyography (EMG) or nerve conduction studies if peripheral neuropathy confirmed; dermatologic consultation for persistent skin lesions.
- Monitoring Frequency: For deficiency diagnosed: retest 4-8 weeks after initiating supplementation; for chronic conditions requiring monitoring: repeat testing every 3-6 months; for migraine sufferers on prophylactic supplementation: annual assessment; for high-risk patients (dialysis, malabsorption): routine monitoring every 6-12 months.
- Fasting Required?
- Fasting Status: NO - Fasting is NOT required for this test. Vitamin B2 quantitative analysis is not significantly affected by food intake or recent meals.
- Patient Preparation: No special preparation needed. Patient may eat and drink normally before blood draw. However, for accurate interpretation, document timing of last supplementation if patient has taken high-dose riboflavin supplements within past 24 hours.
- Medication Considerations: No medications need be withheld; however, inform laboratory of current medications as certain drugs may affect results. Phenothiazines and tricyclic antidepressants may increase riboflavin requirements. Continue all regular medications unless specifically instructed otherwise.
- Specimen Collection Notes: Blood collected in EDTA (ethylenediaminetetraacetic acid) tube, which is lavender-top standard collection tube. Protect sample from light exposure during collection and handling. Specimens should be processed promptly; hemolyzed or lipemic samples may interfere with accurate results and should be recollected.
How our test process works!
