Vitamin D3
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Assesses 25-hydroxyvitamin D levels to determine deficiency, affecting bone strength and immune function.
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🧪 What is Vitamin D3?
Vitamin D3 (cholecalciferol) is a fat-soluble vitamin synthesized in the skin upon sunlight exposure and obtained from dietary sources. In the liver, it is converted to 25-hydroxyvitamin D [25(OH)D], the major circulating form and best indicator of vitamin D status.
❓ Why is the Test Done?
To:
- Assess vitamin D status (deficiency, insufficiency, sufficiency)
- Diagnose causes of bone disorders such as osteomalacia and rickets
- Evaluate patients with osteoporosis, chronic kidney disease, or malabsorption
- Monitor vitamin D supplementation therapy
📊 Normal Range
25(OH) Vitamin D Level | Reference Range |
|---|---|
Deficiency | < 20 ng/mL (< 50 nmol/L) |
Insufficiency | 20–30 ng/mL (50–75 nmol/L) |
Sufficiency | 30–100 ng/mL (75–250 nmol/L) |
Possible toxicity | > 150 ng/mL (> 375 nmol/L) |
📈 Interpretation of Results
Level | Clinical Significance |
|---|---|
Low (<20 ng/mL) | Vitamin D deficiency; risk of bone diseases |
Insufficient (20–30) | Suboptimal vitamin D status |
Sufficient (>30) | Adequate vitamin D for bone health |
High (>100–150) | Risk of vitamin D toxicity and hypercalcemia |
🧠 Associated Conditions
Condition | Details |
|---|---|
Vitamin D deficiency | Causes rickets, osteomalacia, osteoporosis |
Chronic kidney disease | Impaired conversion to active vitamin D |
Malabsorption syndromes | Reduced vitamin D absorption |
Hypervitaminosis D | Excessive supplementation causing toxicity |
🔄 Related / Follow-Up Tests
- Serum calcium and phosphate
- Parathyroid hormone (PTH)
- Vitamin D1,25 dihydroxy (active form)
- Bone mineral density testing
✅ Fasting Required?
Test | Fasting Required |
|---|---|
Vitamin D3 (25(OH)D) | ❌ No |
📝 Summary Table
Parameter | Details |
|---|---|
What | Measures serum 25-hydroxyvitamin D (Vitamin D3 status) |
Why | Assess vitamin D deficiency or sufficiency |
Normal Range | 20–100 ng/mL (deficiency <20 ng/mL) |
Low Levels | Deficiency leading to bone disorders |
Associated Conditions | Rickets, osteomalacia, malabsorption, CKD |
Follow-up Tests | Calcium, PTH, active vitamin D, bone density |
Fasting Required | ❌ No |
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